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- W2364594789 abstract "Objective:Designing dendritic-cell-based vaccines against cancer concerned with the infection of EBV to solve tumor escape. Methods:CD34 + hematopoietic stem cells of bone marrow were cultured in presence of hGM-CSF, hTNF-a, hIL-3, hIL-4 in vitro for two weeks to obtain large amount of DC. DC were characterized by FACS analysis and pulsed with the EBV envelope glycoprotein gp340. The gp340-loaded DC initiate T lymphocytes to induce T lymphocytes activated (CTL) against cancer concerned with the infection of EBV. Results: Marrow-derived DC expressing high levels of GD1a have typical dendritic morphology. They could acquire Epstein-Barr virus latent membrane glycoprotein gp340 efficiently and induce T cells increasing stimulatory capacity in MLR. Only gp340-loaded DC could induce special CTL a-gainst tumor cells concerned with the infection of EBV in cytotoxicity assays. Conclusion: Using DC pulsed with EBV vaccines against EBV infection could start up anti-tumor immunity and induce specific CTLs to availability kill tumor cells associated with EBV in vitro." @default.
- W2364594789 created "2016-06-24" @default.
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- W2364594789 date "2002-01-01" @default.
- W2364594789 modified "2023-09-26" @default.
- W2364594789 title "Induction of Specific Cytotoxic T-lymphocyte Responses Against Tumor Associated with Epstein-Barr Virus Using gp340-Loaded Dendritic Cells Generated from Bone Marrow" @default.
- W2364594789 hasPublicationYear "2002" @default.
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