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- W2364594790 abstract "AIM: To observe the expression of cbfa1 in mouse tooth germs, to clone and sequence the cDNA of cbfa1 from mouse tooth germs. METHODS: Total RNA was isolated from newborn mouse tooth germs tissue and reverse-transcribed into cDNA. The desired DNA product was obtained by PCR with two cbfa1 specific primers. The segment was inserted into pGEM-5zf vector and the positive clone was sequenced by Takara Corporation. RESULTS: cDNA of cbfa1 was obtained from mouse tooth germs tissue. The sequence was consistent with that displayed on Pubmed. No mutation was found. CONCLUSION: This study provides the evidence of expression of cbfa1 in mouse tooth germs tissue." @default.
- W2364594790 created "2016-06-24" @default.
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- W2364594790 date "2002-01-01" @default.
- W2364594790 modified "2023-09-27" @default.
- W2364594790 title "Cloning and sequencing of the coding region of mouse cbfa1 gene" @default.
- W2364594790 hasPublicationYear "2002" @default.
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