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- W2364600809 abstract "Objective To establish a suicide vector-based gene knockout method in studies of Vibrio parahaemolyticus.Methods The upstream and downstream flanking DNA fragments of target gene were fused by PCR,and then cloned into the suicide vector pDS132.The recombinant plasmid was introduced into E.coli strain SM17λpir and transferred into V.parahaemolyticus by conjugation.The sacB gene in pDS132 was sucrose-sensitive and used to screen the deletion mutants.Results The opaR,toxR and aphA mull mutants of V.parahaemolyticus were successfully constructed.Each target gene was exactly deleted,and the mutants constructed were unmarked.Conclusions The gene knockout method is stable with a good efficiency,and can be used for gene function studies of V.parahaemolyticus." @default.
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- W2364600809 date "2011-01-01" @default.
- W2364600809 modified "2023-09-22" @default.
- W2364600809 title "Establishment of a suicide vector-based gene knockout method in studies of Vibrio parahaemolyticus" @default.
- W2364600809 hasPublicationYear "2011" @default.
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