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- W2364607010 abstract "Objective To prepare a kind of gene engineering cells secreting glial cell line-derived neurotrophic factor (GDNF). Methods Mouse GDNF cDNA was amplified from newly-born mouse cortex cell of cerebrum by RT-PCR, and merged into the pEGFP-C1 vector. Then the recombined plasmid was transfected into bone marrow mesenchymal stem cell (MSC). The transfection rate was determined by flow cytometry. Expressions of enhanced green fluorescent protein (EGFP) and GDNF were detected by fluorescence microscope and immunohistochemical technique. Results Plasmid pEGFP/GDNF which was mediated via lipofectamine 2 000 could delivered both EGFP and GDNF gene with high efficiency to MSC. The rate of the transfection of the recombined plasmid into MSC, which was determined by flow cytometry, was 50%. The blight green fluorescence could been observed with fluorescence microscope in pEGFP/GDNF-transfected MSC.GDNF in the engineering cells was strongly positive and it in the others was negative. Conclusion The recombinant eukaryotic expression vector, pEGFP/GDNF can be constructed and expressed efficiently in MSC. EGFP, as a report gene, may reflect expression of GDNF in the engineering cells." @default.
- W2364607010 created "2016-06-24" @default.
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- W2364607010 date "2004-01-01" @default.
- W2364607010 modified "2023-09-29" @default.
- W2364607010 title "Cloning of Glial Cell Line-derived Neurotrophic Factor Gene and its Expression in Bone Marrow Mesenchymal Stem Cell in Mice" @default.
- W2364607010 hasPublicationYear "2004" @default.
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