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- W2364607375 abstract "Aim Orientia tsutsugamushi (R t) are obligate intracellular parasites,and it is difficult to prepare a fair amount of antigens However,we found that Orientia tsutsugamushi could grow extracellularly well when they cultured with Vero E6 cells,and the results indicated that Orientia tsutsugamushi might grow in abiotic culture mediem Orientia tsutsugamushi cultured by the above method were cultured further in the solid medium for 18～24 hours at 36℃ Small colonies,with achromic transparent,or transulent smooth moist sphere or maple,were observed They generated a kind of stench The appearance of colony of Orientia tsutsugamushi J1-90 strain cultured was similar to that of standard Gilliam strain Orientia tsutsugamushi cultured with two methods are used to identify their biological properties,e g morphology staining,pathogenicity to mice,serological specific detection There were no significant differences in the biological properties of Orientia tsutsugamushi cultured by the two methods The results suggested that Orientia tsutsugamushi might have two development phases" @default.
- W2364607375 created "2016-06-24" @default.
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- W2364607375 date "2003-01-01" @default.
- W2364607375 modified "2023-09-28" @default.
- W2364607375 title "Orientia tsutsugamushi developing through two phases" @default.
- W2364607375 hasPublicationYear "2003" @default.
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