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- W2364607565 abstract "In this experiment,Nanog fusion protein was expressed by BL21/pET-32a-Nanog,and the crude Nanog protein was obtained from inclusion body by crushing,high speed centrifuging and washing.Then the inclusion body was dissolved by 8M urea and centrifuging.The supernatant,which be used to Histrap HP column gradual dialysis,renatured and concentrated,was got by centrifuging.the purity of Nanog fusion protein was analyzed by SDS-PAGE and Western blot,and it is up to 97%.The way of preparing mouse Nanog fusion protein from the inclusion body was established,which was prepared for activation detection and mechanism of gene expression and regulation." @default.
- W2364607565 created "2016-06-24" @default.
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- W2364607565 date "2009-01-01" @default.
- W2364607565 modified "2023-09-25" @default.
- W2364607565 title "Purified Recombinant Mouse Nanog Transcription Factor from Inclusion Body" @default.
- W2364607565 hasPublicationYear "2009" @default.
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