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- W2364607888 abstract "OBJECTIVE To study the mutations of LETM1 gene in hepatocellular carcinoma cell lines HepG2.METHODSAfter mRNA abstracted from HepG2 cells,cDNA was synthesized.LETM1 gene was amplified by PCR and connected to vector pCMV-3Myc-2A.The recombination plasmid pCMV-3Myc-LETM1 were confirmed by enzyme digestion and sequencing.RESULTSThe results showed that the recombination plasmid of pCMV-3Myc-LETM1 were constructed,and the point mutations were at G148A(valine→isoleucine),G320A(glycine→asparagine),G344A(arginine→histidine)and A1760G(lysine→arginine).CONCLUSIONThe mitochondria LETM1 gene of HepG2 cells are mutated." @default.
- W2364607888 created "2016-06-24" @default.
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- W2364607888 date "2015-01-01" @default.
- W2364607888 modified "2023-09-23" @default.
- W2364607888 title "Point mutations of LETM1 gene in hepatocellular carcinoma cell lines HepG2" @default.
- W2364607888 hasPublicationYear "2015" @default.
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