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- W2364612416 abstract "Objective To establish an ordinary PCR method to detect 5 kinds of animal origin ingredients, a real time fluorescent PCR method to detect 4 kinds of animal origin ingredients, and serve the food safety. Methods Genomic DNA were extracted from the meat, PCR detection primers and fluorescent probes were synthesized, and the reaction conditions and reaction system were optimized to establish the detection method of animal origin, so as to analyze the meat adulteration in market. Results The measuring method for several kinds animal origin ingredients was established. According to the established method, the adulteration rate for lamb products, donkey products, beef products, and rabbit meat products collected from Baoding area were 75%, 6.7%, 20%, 50%, respectively. The results showed that the adulteration problem existed in Baoding area market, especially in the free market and barbecue samples. The adulteration was serious damage to consumers' rights. Conclusion The established PCR method can obtain DNA quickly, its detection sensitivity can achieve pg or fg level, and is suitable for detecting a large number of meat products." @default.
- W2364612416 created "2016-06-24" @default.
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- W2364612416 date "2014-01-01" @default.
- W2364612416 modified "2023-09-28" @default.
- W2364612416 title "Application of PCR technique for the detection of meat source components" @default.
- W2364612416 hasPublicationYear "2014" @default.
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