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- W2364637039 abstract "Objective To investigate the feasibility of rapid detection of Mycobacterium tuberculosis(MTB) with low-density oligonucleotide membrane-based flow-through hybridization technology.Methods Twelve oligonucleotide probes specific for wild-type and common mutants of the rpoB gene of MTB were designed,and used to prepare the low-density oligonucleotide array.The amplified rpoB gene fragments from MTB isolates by PCR were hybridized with the membrane array by flow-through hybridization technology,and the obtained results were compared with the results of DNA sequencing.Results The results of the drug resistance test and DNA sequencing for 81 MTB isolates showed that the mutations of the rpoB gene existed in 20 of 27(74.1%) rifampicin-resistant isolates.Thirty-one of MTB isolates were detected with low-density oligonucleotide membrane-based flow-through hybridization and the results showed that 17 isolates were rifampicin-resistant mutants and 14 were rifampicin-sensitive wild-type isolates,while DNA sequencing showed 20 isolates were rifampicin-resistant mutants and 11 were rifampicin-sensitive wild-type isolates.Based on the results of DNA sequencing,the sensitivity,specificity,positive predictive value and negative predictive value of the low-density oligonucleotide membrane-based flow-through hybridization technology were 85%(17/20),100%(11/11),100%(17/17) and 78.6%(11/14),respectively.The coincidence rate between the two methods was 90.3%(28/31).Conclusion The low-density oligonucleotide membrane-based flow-through hybridization technology may be used to detect MTB rapidly and easily and should be available to indicate the rifampicin-resistant MTB and guide proper treatment for the patients with TB." @default.
- W2364637039 created "2016-06-24" @default.
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- W2364637039 date "2013-01-01" @default.
- W2364637039 modified "2023-09-23" @default.
- W2364637039 title "Rapid detection of rpoB gene fragment and mutation in Mycobacterium tuberculosis isolates with oligonucleotide membrane-based flow-through hybridization technology" @default.
- W2364637039 hasPublicationYear "2013" @default.
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