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- W2364651637 abstract "Objective:By contrasting 1H-NMR spectrograms of rhamnosylvitexin to examine the spectrogram differences when DMSO-d6 and CD3OD was used as solvent,with the help of this experiment,get some basic data when 1H-NMR was used to determine the quantity of rhamnosylvitexin in hawthorn leaves flavonoids(HLF).Methods:By HPLC to determine the purity of rhamnosylvitexin;By NMR to obtain rhamnosylvitexin′s 1H-NMR spectrograms with different solvents and HLF′s 1H-NMR spectrograms with different internal standard substances.Results:1H-NMR spectrograms peaks′ shape and amount of rhamnosylvitexin were totally different between using DMSO-d6 and CD3OD as solvent.When DMSO-d6 was used as solvent,rhamnosylvitexin standard substance was nearly pure just as the result of HPLC,but when CD3OD was used as solvent,rhamnosylvitexin standard substance was composed of two compounds in fact.With dichlormethane and 3-trimethylsilyl-2,2,3,3-tetradeutero propionic acid(TSP)as internal standard substances,the ratio of relative quantity between two compounds of rhamnosylvitexin was different.Conclusions:1H-NMR spectrogram has a great preponderance in quantity analysis for those uncomplex mixture,especially for those mixture which can′t be separated by HPLC,but the analysis result is affected by solvent and internal-standard substance." @default.
- W2364651637 created "2016-06-24" @default.
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- W2364651637 date "2010-01-01" @default.
- W2364651637 modified "2023-09-27" @default.
- W2364651637 title "Effect of solvent and internal standard substance on rhamnosylvitexin ~1H-NMR spectrograms" @default.
- W2364651637 hasPublicationYear "2010" @default.
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