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- W2364656158 abstract "A forward cDNA-SSH library was established by suppression subtractive hybridization using seedling leaf of Himalaya 10,a drought-tolerant Tibet barley(Hordeum vulgare L.var.nudum HK.f.),among which 600 positive clones were selected for sequencing.after detection by PCR for each clone,each single clone was sequenced.Totally 420 available sequences were assembled and 220 uniESTs,in which 37 contigs and 183 singlets were obtained by cluster analyses of the ESTs.The results of BlastN showed that 189 ESTs had homologous sequences in unigenes database of GenBank,while the other 31 ESTs had no protein homologous.The BlastX results indicated that 158 ESTs had significant homology with proteins encoded by unigenes and 62 were unknown proteins and putative proteins.Most of those genes were not only related to abiotic stresses,but also related to biotic stresses in plants,which suggesting the cross-interactions in response to different stresses in plants,and 58% of the ESTs were homologous with barley ESTs.These UniGenes involved in signal transduction and transcriptional regulation(11.96%),encoding protection,defense and stress protein(9.78%),transport related(5.43%),photosynthesis genes(6.52%),and enzymes for structure and function of metabolic synthesis pathway and other metabolic processes(30.43%).The experimental results indicated that the barley can induce a series of specific expressed genes in the drought-rehydration conditions,not only some known enzymes and protein genes participated in drought response,such as zinc protein,senescence associated protein,CAS,cytochrome P450,heat shock proteins and stress proteins,were found this study,but also some protective protein genes,such as LEA protein,dehydrated protein,P5CS.KOBAS mapped 56 ESTs of the 220 uniESTs to 32 KEGG pathways.This study suggested that the drought tolerance of Tibet barley was closely related with glutamate metabolism,arginine and proline metabolism and Ubiquitin mediated proteolysis." @default.
- W2364656158 created "2016-06-24" @default.
- W2364656158 creator A5033263979 @default.
- W2364656158 date "2012-01-01" @default.
- W2364656158 modified "2023-09-25" @default.
- W2364656158 title "Genes Induced by Drought Stress in Tibetan Hulless Barley(Hordeum vulgare L. var. nudum HK. f.) Ximalaya 10 by Substractive Suppression Analysis" @default.
- W2364656158 hasPublicationYear "2012" @default.
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