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- W2364657708 abstract "A gradient reversed_phase high performance liquid chromatography(RP_HPLC)was developed for the separation of astaxanthin and other pigments from the pigment extracts of the microalga Haematococcus pluvialis.Algal cells were collected by centrifuging the culture fluid at3000g for15min,homogenized and extracted using the solvent mixture of methanol and dichloromethane(3∶1by volume).Chromatographic separation was performed in Nova_Pak C 18 column(300mm×3.9mm ID,5μm)at25℃with an eluent consisting of water,methanol and acetone at a flow rate of1.0mL/min,the eluate was monitored by a photodiode array detector at476nm.Sepˉaration and identification of chlorophylls and multiple carotenoids were completed in30min.The detection limits of astaxanthin,canthaxanthin andβ_carotene were3.56,1.36and29.4μg/L,respectively.The linear ranges of astaxanthin,canthaxanthin andβ_carotene were1.99~31.7mg/L(r=0.9993),2.44~39.0mg/L(r=0.9987)and2.25~36.0mg/L(r=0.9778),respectively.This method is rapid,simple,precise and accuˉrate(RSD0.09%~2.97%,average recoveries96%~102%),and can be used for the quantification of astaxˉanthin and other pigments in Haematococcus Pluvialis." @default.
- W2364657708 created "2016-06-24" @default.
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- W2364657708 date "2003-01-01" @default.
- W2364657708 modified "2023-09-25" @default.
- W2364657708 title "Determination of Astaxanthin and Other Pigments in Haematococcus Pluvialis by HPLC" @default.
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