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- W2364658526 abstract "Objective To investigate the expression of BMP-2 in human periodontal ligament cells(H-PDLCs) after a certain intensity of Low Intensity Pulsed Ultrasound(LIPUS),and to evaluate the osteogenic effect of LIPUS on the periodontium.Methods H-PDLCs were cultured in vitro,and stimulated with LIPUS(90 mW/cm2,20min/day) for one week.The samples were collected on the first day,third day,fifth day,and seventh day after the role of LIPUS.The H-PDLCs were cultured in the same conditions but without LIPUS management as control group.The expression of BMP-2 in H-PDLCs at each time point were detected by Real-time PCR,and the relative gene expression were analyzed by the method of 2^(-△△CT).Statistical analysis was performed by analysis of variance(ANOVA).Results Expression of BMP-2 increased in LIPUS treatment group,reach the peak on day 3,then decreased on day 5,and then dropped to the original level on day 7.Analysis showed that the expression of BMP-2 gene expression increased to 6.07 times on the third day after the role of low Intensity Pulsed Ultrasound,and the expression of BMP-2 gene expression increased to 2.30 times on the fifth day.The differences of △CT between two groups were statistically significant(P0.05).Conclusion This study demonstrates that LIPUS could induce the expression of BMP-2,showing a certain degree of regularity over time.It indicates that LIPUS can promote osteogenic differentiation of H-PDLCs." @default.
- W2364658526 created "2016-06-24" @default.
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- W2364658526 date "2011-01-01" @default.
- W2364658526 modified "2023-10-14" @default.
- W2364658526 title "Effects of Low Intensity Pulsed Ultrasound on the expression of BMP-2 in human periodontal ligament cells" @default.
- W2364658526 hasPublicationYear "2011" @default.
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