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- W2364662788 abstract "Vitamin C is an important antioxidant in plants and also an indispensable nutriment for human beings.In order to improve the content of vitamin C in Lactuca sativa L.,GDP-mannose pyropho-sphorylase(GMP) gene was cloned with specific primers according to the GenBank sequence from cDNA of Arabidopsis thaliana.CaMV 35S promoter,MYC sequence and NOS terminator were connected to GMP gene respectively to constitute a GMP-containing expression cassette,the latter was transferred into pCAMBIA2301 expression vector resulting in p2301-GMP-myc.L.sativa was transformed with Agrobacterium strain EHA105 containing p2301-GMP-myc through Agrobacterium-mediated leaf-disc transformation,and 64 transgenic plants were obtained.PCR and real-time RT-PCR confirmed the integration and expression of the introduced GMP in transgenic L.sativa plants.The contents of vitamin C in transgenic L.sativa plants were measured by HPLC-ELSD.The results indicated that most of the transgenic plants had higher vitamin C contents than wild type plants,with the highest content of vitamin C about 2.5 times of the wild type.This study demonstrated that over-expressing the GMP gene is an effective method to enhance vitamin C content in L.sativa L." @default.
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- W2364662788 date "2011-01-01" @default.
- W2364662788 modified "2023-09-24" @default.
- W2364662788 title "Transformation of GDP-mannose Pyrophosphorylase Gene from Arabidopsis Thaliana L. into Lactuca Sativa L." @default.
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