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- W2364667176 abstract "Objective To obtain an erythromycin high-yield strain by increasing expression of bldD gene in Saccharopolyspora erythraea,and explore the effect of overexpressing bldD gene on the morphological differentiation of Sac.erythraea.Methods Based on Sac.erythraea A226 strain,a mutant A226-△bldD with bldD gene disrupted was constructed by chromosomic homologous recombination method.When expression plasmid pZMW-bldD was introduced into Sac.erythraea A226-△bldD and A226,respectively,A226-△bldD/bldD and A226/bldD strains were obtained.Bacterial suppression test,TCL and HPLC were used to analyze the fermentation products of A226-△bldD/bldD and A226/bldD,and the spore formation process of both strains was observed.Results When the bldD gene was disrupted in Sac.erythraea A226,the obtained Sac.erythraea A226-△bldD produced a lower erythromycin yield,and no spores were formed,while introducing bldD gene into A226-△bldD restored the ability to produce erythromycin and to form spores.It was showed that when bldD gene was introduced into A226,the erythromycin yield increased by 20% and the spore formation occurred earlier compared with Sac.erythraea A226.Conclusion High-yield erythromycin strain of Sac.erythraea could be obtained by overexpressing bldD gene in Sac.erythraea,and adding a bldD gene copy could advance the time of morphological differentiation in Sac.erythraea." @default.
- W2364667176 created "2016-06-24" @default.
- W2364667176 creator A5077871312 @default.
- W2364667176 date "2010-01-01" @default.
- W2364667176 modified "2023-09-23" @default.
- W2364667176 title "Effect of bldD gene overexpression on erythromycin output and spore formation of Saccharopolyspora erythraea" @default.
- W2364667176 hasPublicationYear "2010" @default.
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