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- W2364669532 abstract "Agrobacterium tumefaciens strain EHA105 carrying plasmid P0390 encoding the kanamycin resistance(NPTⅡ) and nicotianamine synthase(NAS) genes was used to transform three Agrostis stolonifera L.varieties.Young embryogenic calli were co-cultured with Agrobacterium in the presence of acetosyringone.Colonies were selected under 40mg/L G418 with cefotaxime,and surviving calli were plated on embryogenesis media.As a result,the target gene was transformed into the embryonic calli and the transformed plants were generated.The Agrobacterium tumefaciens-mediated transfomation efficiencies of different Agrostis stolonifera L.are 0.90%,1.54%,2.02%." @default.
- W2364669532 created "2016-06-24" @default.
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- W2364669532 date "2007-01-01" @default.
- W2364669532 modified "2023-09-26" @default.
- W2364669532 title "Establishment of Agrobacterium tumefaciens-mediated Transformation of Agrostis stolonifera L." @default.
- W2364669532 hasPublicationYear "2007" @default.
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