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- W2364672264 abstract "According to nucleotide sequence of goose paramyxovirus(GPMV)SF02 strain,a pairs of primers was designed and synthesized. The complete HN gene of a domestic isolate DP/02 strain of duck paramyxovirus was amplified by RT-PCR,cloned into pGEMR-T vector sequenced,then explored the genetic variation and prokaryotic expression of the HN gene of DP1/02 strain. The result showed that the HN gene was 1 716 bp long and included a complete open reading frame(ORF)encoding a protein of 571 amino, containing six potential glycosylation sites and thirteen conservative cysteine residues. Compared HN gene of waterfowl paramyxovirus type 1 strains,the homology of the nucleotide and the deduced amino acids are between 81.3%-98.3% and 87.2%-98.4% .Phylogenetic tree of HN nucleotide analysis showed that DP/02 strain has a high evolution level as goose paramyxovirus. The results of SDS-PAGE and Western-blot showed that he molecular weight of the expressed HN protein is about 63 000 and the protein amounted to 30% of the total protein of E.coli BL21(DE3)after induced by IPTG(1 mmol/L)at 37℃ for 3 hours." @default.
- W2364672264 created "2016-06-24" @default.
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- W2364672264 date "2007-01-01" @default.
- W2364672264 modified "2023-09-28" @default.
- W2364672264 title "Genetic variation and prokaryotic expression of HN gene of duck paramyxovirus" @default.
- W2364672264 hasPublicationYear "2007" @default.
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