Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364673776> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2364673776 endingPage "674" @default.
- W2364673776 startingPage "670" @default.
- W2364673776 abstract "Objective To explore the transcription regulation of 5-aza-2'-deoxycytidine(5-Aza-CdR) on SHP-1 gene and its effects on Daudi cell line growth. Methods MTT method and flow cytometry were used to detect the growth and apoptosis of Daudi cells after treated with different dosage of 5-Aza-CdIR. Bisulfite sequencing PCR ( BSP) , T-A cloning and sequence analysis were evaluated for methylation status. The SHP-I mRNA and protein were determined by reverse transcription polymerase chain reaction (RT-PCR) ,immunohistochemistry. Results (1)After 7 d treatment with 2. 00 micromol/L of 5-Aza-CdR, all cytosines (C) in Daudi cells genome DNA were converted to thymidine, and SHP-1 mRNA and protein expressed again in the cells while those Cs in CpG dinucleotides in untreated Daudi cells remained Cs; (2)5-Aza-CdR inhibited the cell growth,The effects within certain extent were dose and time dependent:after 72 h treatment with 5-Aza-CdR at 200. 00, 20. 00, 2. 00 and 0. 20 micromol/L, the inhibitive rates were 72. 0% , 65. 1%, 51. 5%, 28.8% ,23.4% respectively; (3) 5-Aza-CdR increased apoptosis rate of tumor cells with a dose and times dependent manner within certain extent, too:at the 1,3,5 d treatment with 5-Aza-CdR 2. 00 micromol/L,the apoptosis rates were 2. 3% ,10. 8 % and 17. 1% ; respectively. (4) 5-Aza-CdR also changed cell cycle of tumor cells: at 24 h treatment with 5-Aza-CdR 2.00 micromol/L,92. 7% tumor cells stopped at S phase and G, phase cells were increased gradually with time. Conclusion DNA promoter hypermethylation is associated with SHP-1 gene silence in Daudi lymphoma cell line. 5-Aza-CdR could effectively cause demethylation and inhibit the growth of tumor cell by reactivating the gene transcription." @default.
- W2364673776 created "2016-06-24" @default.
- W2364673776 creator A5007218112 @default.
- W2364673776 creator A5020056891 @default.
- W2364673776 creator A5027475930 @default.
- W2364673776 creator A5038566539 @default.
- W2364673776 creator A5062092477 @default.
- W2364673776 creator A5065697046 @default.
- W2364673776 creator A5067974635 @default.
- W2364673776 creator A5069807545 @default.
- W2364673776 date "2006-10-01" @default.
- W2364673776 modified "2023-09-25" @default.
- W2364673776 title "[SHP-1 gene's methylation status of Daudi lymphoma cell and the demethylation effect of 5-aza-2'-deoxycytidine]." @default.
- W2364673776 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17343198" @default.
- W2364673776 hasPublicationYear "2006" @default.
- W2364673776 type Work @default.
- W2364673776 sameAs 2364673776 @default.
- W2364673776 citedByCount "0" @default.
- W2364673776 crossrefType "journal-article" @default.
- W2364673776 hasAuthorship W2364673776A5007218112 @default.
- W2364673776 hasAuthorship W2364673776A5020056891 @default.
- W2364673776 hasAuthorship W2364673776A5027475930 @default.
- W2364673776 hasAuthorship W2364673776A5038566539 @default.
- W2364673776 hasAuthorship W2364673776A5062092477 @default.
- W2364673776 hasAuthorship W2364673776A5065697046 @default.
- W2364673776 hasAuthorship W2364673776A5067974635 @default.
- W2364673776 hasAuthorship W2364673776A5069807545 @default.
- W2364673776 hasConcept C104317684 @default.
- W2364673776 hasConcept C121608353 @default.
- W2364673776 hasConcept C140173407 @default.
- W2364673776 hasConcept C150194340 @default.
- W2364673776 hasConcept C153911025 @default.
- W2364673776 hasConcept C185592680 @default.
- W2364673776 hasConcept C189235521 @default.
- W2364673776 hasConcept C190283241 @default.
- W2364673776 hasConcept C190727270 @default.
- W2364673776 hasConcept C2777844706 @default.
- W2364673776 hasConcept C2779277951 @default.
- W2364673776 hasConcept C2780258809 @default.
- W2364673776 hasConcept C29537977 @default.
- W2364673776 hasConcept C33288867 @default.
- W2364673776 hasConcept C502942594 @default.
- W2364673776 hasConcept C54355233 @default.
- W2364673776 hasConcept C553184892 @default.
- W2364673776 hasConcept C55493867 @default.
- W2364673776 hasConcept C62112901 @default.
- W2364673776 hasConcept C86803240 @default.
- W2364673776 hasConceptScore W2364673776C104317684 @default.
- W2364673776 hasConceptScore W2364673776C121608353 @default.
- W2364673776 hasConceptScore W2364673776C140173407 @default.
- W2364673776 hasConceptScore W2364673776C150194340 @default.
- W2364673776 hasConceptScore W2364673776C153911025 @default.
- W2364673776 hasConceptScore W2364673776C185592680 @default.
- W2364673776 hasConceptScore W2364673776C189235521 @default.
- W2364673776 hasConceptScore W2364673776C190283241 @default.
- W2364673776 hasConceptScore W2364673776C190727270 @default.
- W2364673776 hasConceptScore W2364673776C2777844706 @default.
- W2364673776 hasConceptScore W2364673776C2779277951 @default.
- W2364673776 hasConceptScore W2364673776C2780258809 @default.
- W2364673776 hasConceptScore W2364673776C29537977 @default.
- W2364673776 hasConceptScore W2364673776C33288867 @default.
- W2364673776 hasConceptScore W2364673776C502942594 @default.
- W2364673776 hasConceptScore W2364673776C54355233 @default.
- W2364673776 hasConceptScore W2364673776C553184892 @default.
- W2364673776 hasConceptScore W2364673776C55493867 @default.
- W2364673776 hasConceptScore W2364673776C62112901 @default.
- W2364673776 hasConceptScore W2364673776C86803240 @default.
- W2364673776 hasIssue "10" @default.
- W2364673776 hasLocation W23646737761 @default.
- W2364673776 hasLocation W23646737762 @default.
- W2364673776 hasOpenAccess W2364673776 @default.
- W2364673776 hasPrimaryLocation W23646737761 @default.
- W2364673776 hasRelatedWork W1588226643 @default.
- W2364673776 hasRelatedWork W2008002281 @default.
- W2364673776 hasRelatedWork W2029970181 @default.
- W2364673776 hasRelatedWork W2348771488 @default.
- W2364673776 hasRelatedWork W2349146475 @default.
- W2364673776 hasRelatedWork W2350723360 @default.
- W2364673776 hasRelatedWork W2352751940 @default.
- W2364673776 hasRelatedWork W2357351754 @default.
- W2364673776 hasRelatedWork W2361950558 @default.
- W2364673776 hasRelatedWork W2373617593 @default.
- W2364673776 hasRelatedWork W2377048160 @default.
- W2364673776 hasRelatedWork W2378274563 @default.
- W2364673776 hasRelatedWork W2380235902 @default.
- W2364673776 hasRelatedWork W2386737794 @default.
- W2364673776 hasRelatedWork W2390703260 @default.
- W2364673776 hasRelatedWork W2396108596 @default.
- W2364673776 hasRelatedWork W2428969339 @default.
- W2364673776 hasRelatedWork W3029625390 @default.
- W2364673776 hasRelatedWork W3031459849 @default.
- W2364673776 hasRelatedWork W3032228356 @default.
- W2364673776 hasVolume "27" @default.
- W2364673776 isParatext "false" @default.
- W2364673776 isRetracted "false" @default.
- W2364673776 magId "2364673776" @default.
- W2364673776 workType "article" @default.