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- W2364683207 abstract "The gene fragment of outer membrane lipoprotein(OML) was amplified from genomic DNA of Actinobacillus pleuropneumoniae(APP) strain SC-A by PCR and cloned into pMD18-T vector.Plasmid DNA was extracted and digested with enzymes and sequenced to confirm its rightness.The OML gene was subcloned into the expression vector pET-32a(+),and the recombinant plasmid was named as pET-mOML.The plasmid pET-mOML was transformed into E.coli BL21 and expressed under the induction of 1.0 mmol/L IPTG.The results of SDS-PAGE showed a protein band with an approximate molecular weight of 60 ku.The fusion protein could react with mice serum against mOML,as confirmed by ELISA.In conclusion,the OML gene was highly expressed in the prokaryotic system and the expressed protein had a good immunogenicity." @default.
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- W2364683207 date "2008-01-01" @default.
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- W2364683207 title "Prokaryotic expression and immunogenicity analysis of outer membrane lipoprotein of Actinobacillus pleuropneumoniae" @default.
- W2364683207 hasPublicationYear "2008" @default.
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