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- W2364692009 abstract "Objective To construct the eukaryotic expression recombinant plasmid expressing Mammaglobin A and Lipophilin B and to establish human breast cancer T47-D cell lines stable expression Mammaglobin A and stable co-expression Mammaglobin A and Lipophilin B.Methods The Mammaglobin A and Lipophilin B genes were cloned from human breast cancer cell line MDA-MB-415,the eukaryotic expression vectors pc-MA,pc-LP were constructed and then transfected in to T47-D cells.The positive cells were screening by G418 and examined by RT-PCR and Western Blotting analysis.Results Recombinant plasmids expressed Mammaglobin A and Lipophilin B were successfully constructed confirmed by restriction enzyme digestion and DNA sequencing analysis.Stable expression of ectopic Mammaglobin A and Lipophilin B in T47-D cells were examined by RT-PCR and Western Blot analysis.Conclusion T47-D Cell line which stably expressed ectopic Mammaglobin A and Lipophilin B is successfully established.It provides a model for studying the function of Mammaglobin A and Lipophilin B in the breast carcinogenesis." @default.
- W2364692009 created "2016-06-24" @default.
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- W2364692009 date "2010-01-01" @default.
- W2364692009 modified "2023-09-23" @default.
- W2364692009 title "Co-transfection of Mammaglobin A and Lipophilin B in breast cancer T47-D cells" @default.
- W2364692009 hasPublicationYear "2010" @default.
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