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- W2364692937 abstract "Human hepatitis B virus(HBV)DNA was detected by an objective and quantitative ploymerase chain reaction based on the enzyme linked immunosorbent assay(PCR ELISA).The specific probe labelled with biotin was added into the streptavidin coated wells,the biotinylated probe was captured;The primers of PCR were modified with digoxin so that the amplified products were labelled,followed by denaturation of the hot,and added into the probe coated wells,then hybridization was performed;Finally anti digoxin AKP conjugates were added,After washed,P NPP was added into the wells and then measured on a microplate reader at 405 nm.The best conditions of PCR-ELISA were:appropriate cycle number of amplification was30;Hybridization temperature was 37 ℃;hybridization time was 1 h;ELISA detection of amplified products of HBV DNA was showed that this assay was rapid,sensitive specific and accurate.The correlation between the initial number of viral template and ELISA results of amplified products was good.The positive limitation(cut off)was 0 134.The within run CV was 9 4%,the between run CV was 16 9%,the positive percentage of serum HBV DNA in 60 cases of hepatitis B patients were 70%(42/60)and 46 7%(28/60)respectively by ELISA and 20 g/L agarose electrophoresis,they are obvious difference(P0 01)." @default.
- W2364692937 created "2016-06-24" @default.
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- W2364692937 date "2000-01-01" @default.
- W2364692937 modified "2023-10-08" @default.
- W2364692937 title "Detection of the human hepatitis B virus in serum by polymerase chain reaction based on enzyme linked immunsorbent assay" @default.
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