Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364696445> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2364696445 abstract "In order to study single viral gene functions in the context of genome and analysis interactions between one gene with another, the HEK293/p2089 stable cell line was established by transfecting the plasmid of DNA (p2089) into EBV-negative 293 cells and selecting for resistance against hygromycin. The plasmid p2089, which was kindly provided by Prof.Hammerschmidt, contained the whole EBV genome of wild-type B95-8. Two eukaryotic expression vectors (pcDNA3.1(+)/BZLF1 and pcDNA3.1(+)/BALF4) were constructed and then transiently cotransfected into the HEK293/p2089 stable cells, so as to induct EBV lytic replication and product recombinant EBV particles visualized through GFP-expressing. To estimate the EBV production, Raji cells were incubated with supernatants from the induced 293 cells carrying p2089 DNA, as revealed by indirect visualization of the Raji cells. GFP-positive cells were evaluated by inverted fluorescence microscope or FACS analysis. The different virus supernatants were quantified with the help of green Raji units per ml as an absolute number of infectious particles. This technique makes it possible for the reconstitution of viral progeny or mutants by transfection of BAC plasmid into eukaryotic cells, and any genetic modification in E. coli, thereby facilitating the analysis of viral gene functions in the context of genome. This new technique has provided a useful tool for the study of pathogenesis mechanism of EBV, especially for that of cancer-associated." @default.
- W2364696445 created "2016-06-24" @default.
- W2364696445 creator A5052049405 @default.
- W2364696445 date "2007-01-01" @default.
- W2364696445 modified "2023-09-24" @default.
- W2364696445 title "Lytic Replication and Inductive Production of Recombinant Epstein Barr Virus Visualized" @default.
- W2364696445 hasPublicationYear "2007" @default.
- W2364696445 type Work @default.
- W2364696445 sameAs 2364696445 @default.
- W2364696445 citedByCount "0" @default.
- W2364696445 crossrefType "journal-article" @default.
- W2364696445 hasAuthorship W2364696445A5052049405 @default.
- W2364696445 hasConcept C104317684 @default.
- W2364696445 hasConcept C140704245 @default.
- W2364696445 hasConcept C153911025 @default.
- W2364696445 hasConcept C159047783 @default.
- W2364696445 hasConcept C16232356 @default.
- W2364696445 hasConcept C174510640 @default.
- W2364696445 hasConcept C182615771 @default.
- W2364696445 hasConcept C22744801 @default.
- W2364696445 hasConcept C2522874641 @default.
- W2364696445 hasConcept C2776409557 @default.
- W2364696445 hasConcept C2780727368 @default.
- W2364696445 hasConcept C2781295983 @default.
- W2364696445 hasConcept C54009773 @default.
- W2364696445 hasConcept C54355233 @default.
- W2364696445 hasConcept C81885089 @default.
- W2364696445 hasConcept C86803240 @default.
- W2364696445 hasConceptScore W2364696445C104317684 @default.
- W2364696445 hasConceptScore W2364696445C140704245 @default.
- W2364696445 hasConceptScore W2364696445C153911025 @default.
- W2364696445 hasConceptScore W2364696445C159047783 @default.
- W2364696445 hasConceptScore W2364696445C16232356 @default.
- W2364696445 hasConceptScore W2364696445C174510640 @default.
- W2364696445 hasConceptScore W2364696445C182615771 @default.
- W2364696445 hasConceptScore W2364696445C22744801 @default.
- W2364696445 hasConceptScore W2364696445C2522874641 @default.
- W2364696445 hasConceptScore W2364696445C2776409557 @default.
- W2364696445 hasConceptScore W2364696445C2780727368 @default.
- W2364696445 hasConceptScore W2364696445C2781295983 @default.
- W2364696445 hasConceptScore W2364696445C54009773 @default.
- W2364696445 hasConceptScore W2364696445C54355233 @default.
- W2364696445 hasConceptScore W2364696445C81885089 @default.
- W2364696445 hasConceptScore W2364696445C86803240 @default.
- W2364696445 hasLocation W23646964451 @default.
- W2364696445 hasOpenAccess W2364696445 @default.
- W2364696445 hasPrimaryLocation W23646964451 @default.
- W2364696445 hasRelatedWork W1484203766 @default.
- W2364696445 hasRelatedWork W1510528005 @default.
- W2364696445 hasRelatedWork W1517670419 @default.
- W2364696445 hasRelatedWork W1598480190 @default.
- W2364696445 hasRelatedWork W1617966488 @default.
- W2364696445 hasRelatedWork W1619023374 @default.
- W2364696445 hasRelatedWork W1829111599 @default.
- W2364696445 hasRelatedWork W1841083068 @default.
- W2364696445 hasRelatedWork W1946287511 @default.
- W2364696445 hasRelatedWork W1957171527 @default.
- W2364696445 hasRelatedWork W1970475152 @default.
- W2364696445 hasRelatedWork W2023813489 @default.
- W2364696445 hasRelatedWork W2025237513 @default.
- W2364696445 hasRelatedWork W2026415334 @default.
- W2364696445 hasRelatedWork W2098573136 @default.
- W2364696445 hasRelatedWork W2113880258 @default.
- W2364696445 hasRelatedWork W2118426628 @default.
- W2364696445 hasRelatedWork W2155610307 @default.
- W2364696445 hasRelatedWork W2309397256 @default.
- W2364696445 hasRelatedWork W72938498 @default.
- W2364696445 isParatext "false" @default.
- W2364696445 isRetracted "false" @default.
- W2364696445 magId "2364696445" @default.
- W2364696445 workType "article" @default.