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• W2364700390 abstract "There are unique geographical and climatological characteristics in the Arctic and Antarctic areas.Only during short summer time ice and snow melt in some region,both areas are covered by ice and snow all the year round.Highly variable illumination radiation,seasonal sunshine time and extremely low temperature result in unique biological characteristics of the polar microorganism.Since the Ekelof's report on the first isolation of microorganism from the Antarctic in 1908,many microbiologists from different countries did numerous work successively in the polar areas and confirmed that polar microorganisms have broad prospects in fundamental research,exploitation and application.Therefore the polar region is regarded as a potential and important microorganism resource base,and may become a potential source place of new types of active substance and forerunner compounds.The strain AFN2001 is an actinomycete isolated from the lake sediment of the Arctic area.Fermenting in the basal fermentation broth FI(KNO3 1.0 g,Soluble starch 20.0 g,K2HPO4 0.5 g,MgSO4 0.5 g,NaCl 0.5 g,H2O 1 000 mL,pH 7.2~7.4),the metabolites of AFN2001 possesses evident inhibition on Staphylococcus aureus(ATCC 25923,the diameter of the inhibiying circle was 23 mm),but no inhibition on Pseudomonas aeruginosa(ATCC 25916) and Escherichia coli(ATCC 25922).In order to find out suitable nutrients required for generating antibacterial activated substance against Staphylococcus aureus,various carbon sources(glucose,sucrose,starch,2%,w/v) or nitrogen sources(yeast extract,casin,peptone,NaNO3,KNO3,NH4NO3,NH4Cl,2%,w/v) were respectively used in the basal medium with the one-factor-at-a-time method.The results show that sucrose and starch is the suitable carbon source for generating antibacterial activated substance with AFN2001,while soybean extract,yeast extract,NaNO3 and KNO3 is suitable nitrogen source.In which soybean extra has evident superiority.Thus,4 nutrient material(starch,sucrose,soybean extract,NaNO3) were selected for the orthogonal matrix.Orthogonal optimization tests at tree levels with seven nutrients(starch,soybean extract,sucrose,NaNO3,MgSO4,K2HPO3 and water quality) were made to get the maximum antibacterial activated substance,and visual and variance analyses were carried out on the results,which show that they could be ranked in importance as NaNO3starchK2HPO3 MgSO4soybean extractsucrosewater quality.The optimized nutrient medium FII is starch 10 g,sucrose 10 g,soybean extract 6 g,MgSO4 0.5 g,tap water 1 L.To determine the optimum fermentation condition,the optimized medium FII was used as the nutrient medium and the orthogonal design L9(34) was used to optimize the fermentation temperature(A),starting pH value(B) and volume of broth(C).Visual and variance analyses of the results show that the factors could be ranked in importance as temperaturepH valuevolume,the optimal combination is A2B1C1 or A2B1C3 and the optimal condition of fermentation is 28 ℃,pH 6,and 250/500 mL flask.The optimized medium is starch 10 g,sucrose 10 g,soybean extract 6 g,MgSO4 0.5 g and tap water 1 L.AFN2001 strain cultivation with the basal medium and conditions and optimized medium and conditions shows that the maximum bacteria inhibition circle was 22 mm on the third day when FI basal medium was used,while it was 36 mm when FII optimized medium was used,which is 1.64 times higher than that with the basal medium.This shows that the optimized medium and cultivation conditions from the orthogonal experiment can significantly raise the activity of AFN2001 fermentation fluid and thus make it possible to isolate antibacterial activated substance from Arctic actinomycete." @default.
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• W2364700390 date "2008-01-01" @default.
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• W2364700390 title "Optimization of the media and fermentation conditions of arctic actinomycete AFN2001" @default.
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