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- W2364706947 abstract "Objective To study the significance of the correlation between FOXP3 mRNA in mononuclear cells and CD4+CD25+Treg. Methods Total RNA was extracted with Trizol from human peripheral blood mononuclear cells(PBMC) and mRNA was transcribed reversely into cDNA. The real-time fluorescence quantitative RT-PCR was used to detect the expression of FOXP3 mRNA in 29 pediatric patients with asthma and 24 healthy children with β-actin as the internal control gene. The specificity of FOXP3 and β-actin amplifications was confirmed by analyzing the dissociation curves and electrophoresis of the target amplicon. Flow cytometry analysis was performed to assess the percentage of CD4+CD25+ regulatory T cells. Results The percentage of CD4+CD25+ regulatory T cells and FOXP3 mRNA expression of the asthma children was significantly lower than that of the healthy children(P0.01, P0.05) respectively. The specificities of FOXP3 and β-actin amplifications were confirmed by analyzing the dissociation curves and electrophoresis of PCR products with the values of Tm 82.4℃ and 87.8℃, respectively. Conclusions Quantitative detection of FOXP3 mRNA by the method of SYBR GreenⅠreal-time fluorescence quantitative RT-PCR is stable and reliable. The decrease of CD4+CD25+ regulatory T cells may be involved in the pathogenesis of asthma. The decreased mRNA expression of FOXP3 may be associated with the aberrant development of CD4+CD25+ regulatory T cells." @default.
- W2364706947 created "2016-06-24" @default.
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- W2364706947 date "2008-01-01" @default.
- W2364706947 modified "2023-09-28" @default.
- W2364706947 title "The significance of the correlation between FOXP3 mRNA in mononuclear cells and CD4~+CD25~+Treg" @default.
- W2364706947 hasPublicationYear "2008" @default.
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