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- W2364720352 abstract "Objective To construct the eukaryotic expression plasmid pIRES-neo-G250 which expresses human G250,transduce it into the 293T cell and detect its expression.Methods The human G250 gene was amplified by PCR and linked into PMD18T,which was then inserted into the eukaryotic expression vector pIRES-neo.The constructed recombinant plasmid pIRES-neo-G250 was tansfected into the 293T cells,and its expression was determined by FACS,IMF and RT-PCR.Results The gene sequence of human G250 which was amplified by PCR was right.Enzyme digestion analysis revealed that the recombinant plasmid pIRES-neo-G250 was successfully constructed.FACS,IMF and RT-PCR revealed that the recombinant plasmid was expressed in 293T cells.Conclusion The successful construction of recombinant plasmid pIRES-neo-G250 and its effective expression in 293T cells can serve further construction of human G250-transfected cell line." @default.
- W2364720352 created "2016-06-24" @default.
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- W2364720352 date "2013-01-01" @default.
- W2364720352 modified "2023-09-28" @default.
- W2364720352 title "Construction and expression of recombinant plasmid pIRES-neo-G250 expressing human G250" @default.
- W2364720352 hasPublicationYear "2013" @default.
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