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- W2364722080 abstract "To done and express the gene coding dense granule protein 4 (GRA4) of Toxoplasma gondii RH strain, a pair of primers were designed and synthesized, according to the sequence of GRA4 gene, by using PCR technique, and the specific fragment of GRA4 gene was obtained by amplification of genomic DNA of Toxoplasma gondii RH strain. After purification, the fragments of PCR products were cloned into pMD18-T vector, then transferred into E. coli JM109. Positive clones were identified by PCR, restrictive enzyme digestion and sequence analysis. The inserted GRA4 gene was subcloned into pGEX-4T-2 vector, a recombinant pGEX-4T-2-GRA4 was constructed. The fusion recombinant protein was expressed in E. coli BL21 and was analyzed by means of SDS-PAGE and Western blotting.The specific gene fragment GRA4 was amplified form genomic DNA of Toxoplasma gondii RH strain, and the recombinant plasmid pGEX-4T-2-GRA4 could express a fusion protein in E. coli BL21 which could be recognized by immune rabbit serum.The recombinant plasmid which contain the gene fragment encoding GRA4 of T. gondii has been successfully constructed and expressed.This provides the basis for the diagnosis and vaccination against Toxoplasmosis." @default.
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- W2364722080 date "2004-01-01" @default.
- W2364722080 modified "2023-09-26" @default.
- W2364722080 title "Cloning and expression of GRA4 gene of Toxoplasma gondii RH strain" @default.
- W2364722080 hasPublicationYear "2004" @default.
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