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- W2364749781 abstract "The reactive oxygen species( ROS) play an important role in plants which act as intracellular or intercellular signals. But high concentration of ROS might lead to plant male sterility. Glutathione S-transferases( GSTs) are crucial to the detoxification for the cells. Based on comparative transcriptome analysis between the cytoplasmic-nuclear male sterile line NJCMS1 A and its maintainer NJCMS1 B in Glycine max,a differential expression gene of Gm GST12 was identified in our previous research. In the present study,Gm GST12 gene was cloned from the flower buds of NJCMS1 A and NJCMS1 B by homologybased cloning method. The coding DNA sequences( CDSs) of Gm GST12 from NJCMS1 A and NJCMS1 B were 708 bp long with the same nucleotide sequences and coded the glutathione S-transferases protein consisted of 235 amino acids. Phylogenetic analysis showed that the homology between Gm GST12 and At GSTU9 from Arabidopsis thaliana was the highest and the identity of the amino acid sequences was 52%. The tissues expression analysis showed that the mRNA expression level of Gm GST12 in flower buds of NJCMS1 A was significantly higher than that in flower buds of NJCMS1 B,but no difference was found in root,steam and leaf. The result of subcellular localization indicated that Gm GST12 was located in the cytoplasm and nucleus. In addition,the plant overexpression vector p CAMBIA3301-Gm GST12 was constructed for further transgenic research. All the above results provided the foundation for future study on the molecular mechanism of cytoplasmic-nuclear male sterility in soybean." @default.
- W2364749781 created "2016-06-24" @default.
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- W2364749781 date "2015-01-01" @default.
- W2364749781 modified "2023-09-28" @default.
- W2364749781 title "Cloning and Expression Analysis of GmGST12 in Soybean" @default.
- W2364749781 hasPublicationYear "2015" @default.
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