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- W2364752676 abstract "Objective: To develop a rapid and sensitive method for detecting MBL genetic mutants and evaluate the association with recurrent respiratory tract infections(RRTI)in children.Methods: The MBL exon-1 region was amplified by PCR,the non-incorporated primers and excesses dNTPs were removed by enzymatic digestion.The dye-terminator(R110-acyC or TAMRA-acyT) was incorporated on the 3′-end of the primer via template directed dye-terminator incorporation reaction(TDI) and the mutations were determined by fluorescence polarization(FP) assay.The MBL mutants were analyzed among 50 healthy and 46 RRTI children using this new method.Results: The accuracy and sensitivity were evaluated and verified by standard sequencing method.The 54 GA mutant was found among both healthy and RRTI children.The genotypes among healthy group were: 39 homozygote wild-type G/G(78.00%),8 heterozygote G/A(16.00%) and 3 homozygote mutant A/A(6.00%).The genotypes among the RRTI children were: 18 homozygote wild-type G/G(39.13%),22 heterozygote G/A(47.83%) and 6 homozygote mutant T/T(13.14%).The frequency of MBL mutation was significant higher in RRTI children than in healthy controls.The 52CT and 57GA mutants were not found in both groups.Conclusions: The MBL mutation associates with the risk of RRTI among children.This new MBL genotype can be used to help the diagnosis and screening the high risk children for RRTI." @default.
- W2364752676 created "2016-06-24" @default.
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- W2364752676 date "2010-01-01" @default.
- W2364752676 modified "2023-09-24" @default.
- W2364752676 title "Development of a fluorescence polarization method for rapid detecting the MBL mutants and the detection in recurrent respiratory tract infections children" @default.
- W2364752676 hasPublicationYear "2010" @default.
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