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- W2364756897 abstract "An indirect enzyme-linked immunosorbent assay(ELISA) was standardized to detect the antibody against follicular-stimulating hormone(FSH).Being tested with the indirect ELISA,5 serum samples which were acquired by the FSH-BSA immunized BaLb/cmouses were all positive.The optimum work concentration of the indirect ELISA was established.Polystyrene micro-titer plates were coated by the purified FSH at theconcentration of 10?μg/ml.The working concentration of serum samples was 1∶500 and the sheep-against-mice IgG labeled by HRP was 1∶1?200.The confining liquid was 1.0% defatted milk powder.Antigenblockage test was designed to check the results." @default.
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- W2364756897 date "2006-01-01" @default.
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- W2364756897 title "Establishment of the Detection of the Antibody against Follicular-stimulating Hormone with the Indirect ELISA" @default.
- W2364756897 hasPublicationYear "2006" @default.
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