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- W2364757007 abstract "Objective:To explore the application value of fluorescence in situ hybridization(FISH) by using self-designed human whole chromosome 21 special DNA probe for prenatal diagnosis of Down's syndrome after artificial insemination by husband(AIH).Methods:FISH of uncultured amniotic fluid cells Abstracted from pregnant women of 16-26 gestational weeks after AIH treatment was performed with self-designed human whole chromosome 21 special DNA probe,routine cell culture and chromosomal karyotype analysis were conducted at the same time,and the results of the two methods were compared.Results:The result of FISH was obtained within 24 hours,one child with trisomy 21 and one child with triple X syndrome were found.The coincidence rate of self-designed human whole chromosome 21 special DNA probe for detection of chromosome 21 in uncultured amniotic fluid cells was as high as 99.42%,no abnormal FISH result was found in the patients whose chromosomal karyotype was 47,XXX.The detection results were identical with the results of chromosomal karyotype analysis and follow-up.Conclusion:FISH technique with self-designed human whole chromosome 21 special DNA probe has the advantages of speediness and accuracy,which can quicken the diagnostic time,FISH technique has good application value for the high risk pregnant women after successful AIH in the prenatal diagnosis of Down's syndrome." @default.
- W2364757007 created "2016-06-24" @default.
- W2364757007 creator A5001985176 @default.
- W2364757007 date "2012-01-01" @default.
- W2364757007 modified "2023-09-24" @default.
- W2364757007 title "Application of FISH technique with whole chromosome probe for prenatal diagnosis of Down's syndrome after AIH" @default.
- W2364757007 hasPublicationYear "2012" @default.
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