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- W2364757674 abstract "[Objective]To examine the difference of proliferation and α-smooth muscle actin(α-SMA) expression between healing and normal medial collateral ligament(MCL) fibroblasts.[Method]Ten adult,male,Sprague-Dawley rats weighing between 350 and 375 g were used in this study.Normal and healing MCL were cut into small pieces in aseptic conditions,and then placed and cultured in culture chamber.Fibroblasts were passaged with 0.25% trypsin.After 24 and 48 hours incubation,MTT was used to measure the cell proliferation.The production of α-SMA was measured by Western Blot.[Result]After 24 and 48 hours incubation,healing fib roblasts showed absorbency values of 0.49±0.080 and 0.53±0.07 respectively.II was found that healing fibroblasts proliferated faster than normal fibroblasts(P0.05).Healing fibroblasts had 1.7 fold greater protein expression of a-SMA than normal fibroblasts(P0.05).[Conclusion]Healing fibroblasts possess a faster proliferation and higher a-SMA protein expression than normal fibroblasts.The findings provide a cellular and molecular basis for realizing the mechanism and improving the quality of healing process." @default.
- W2364757674 created "2016-06-24" @default.
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- W2364757674 date "2008-01-01" @default.
- W2364757674 modified "2023-09-23" @default.
- W2364757674 title "Biological differences between healing and normal medial collateral ligament fibroblasts" @default.
- W2364757674 hasPublicationYear "2008" @default.
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