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- W2364759098 abstract "Objective To set up the reliable genotyping of human platelet antigens (HPA) of 1,2,3,4,5,6 system by PCR- SSP. Methods In this study, 18 sequence- specific primers were designed and synthesized. The concentration of each primer pair, the concentra- tion of Mg2+ and the PCR conditions were adjusted to the optimum so that HPA- 1 to 6 sys- tems could be amplified simultaneously under the same PCR cycling parameters. The accura- cy and reliability of the assay evaluated was evaluated and confirmed by typing the coded DNA samples distributed by the 10TH and 11TH Platelet Genotyping Workshop of the Interna- tional Society of Blood Transfusion (ISBT). A total of 198 volunteer platelet donors in Shen- zhen wrere genotyped at HPA- 1 to HPA- 6 systems. Results The coded samples distributed by the 10TH and 11TH Platelet Genotyping Workshop of ISBT were genotyped by PCR- SSP method, a concordance rate of 100 percent was observed between our results and the results provided by ISBT report. The HPA gene frequencies observed in 198 randomly- selected healthy platelet dornors in Shen zhen were 0.9924 and 0.0076 for HPA- 1a and 1b, 0.9545 and 0.0455 for HPA- 2a and 2b, 0.5556 and 0.4444 for HPA- 3a and 3b, 0.9975 and 0.0025 for HPA- 4a and 4b, 0.9848 and 0.0152 for HPA- 5a and 5b, 0.9798 and 0.0202 for HPA- 6a and 6b respectively. Conclusions PCR- SSP assay provided a simple, rapid and accurate method for HPA genotyping, it can be used in routine clinical HPA genotyping and shows a broad prospect in its further applications." @default.
- W2364759098 created "2016-06-24" @default.
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- W2364759098 date "2005-01-01" @default.
- W2364759098 modified "2023-09-25" @default.
- W2364759098 title "Study on the genotyping of human platelet antigens of 1,2,3,4,5,6 system by PCR-SSP" @default.
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