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- W2364767522 abstract "To aim at the feasibility of infectious cDNA clones of PRRSV as foreign sequence expression vectors,and find out why PRRSV delete the foreign sequence.Based on our established infectious clone of PRRSV,designated as pAPRRS,the full-length cDNA pDCPV containing the 108 nt deleted from 3 773 nt to 3 882 nt in nsp2 and PCV-2 ORF2 inserted between ORF1 and ORF2.The full length mutant clones pDCPV was transfected into Marc-145 cells,and the rescued virus vDCPV was gained.The study on the genetically stability of the rescued virus vDCPV showed vDCPV was more stable than vCPV only containing PCV-2 ORF2 inserted between ORF1 and ORF2 reported,which indicated that the mechanism of deleting foreign sequence was related to the full length of the genome containing the foreign sequences,possibly for the too long genome may influence the package of N protein and other structure protein.Cap protein can be expressed slightly for vDCPVP identified by IFA." @default.
- W2364767522 created "2016-06-24" @default.
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- W2364767522 date "2012-01-01" @default.
- W2364767522 modified "2023-09-24" @default.
- W2364767522 title "Study on Expression of Infective PRRSV Clones with PCV-2 ORF2 in Marc-145 Cells" @default.
- W2364767522 hasPublicationYear "2012" @default.
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