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- W2364777640 abstract "Phosphoenolpyruvate synthase (PpsA) and phosphoenolpyruvate carboxykinase (PckA), which are encoded by pps A and pck A genes in Escherichia coli, are the key enzymes for biosynthesis of phosphoenolpyruvate (PEP) in the pathway of carbon center metabolism. In this work, pps A and pck A genes were amplified from genomic DNA of E. coli K12 by polymerase chain reaction (PCR). The two genes were introduced into p λ P R plasmid either independently or in a tandem way. The resulted constructs p λ P R ppsA, pλP R pckA and p λ P R ppsA pckA were transformed into the E. coli P2392 strain for expression. The activity of PpsA in the transformed cells harboring p λ P R ppsA increased 5.2 fold. The activity of PckA in E. coli cells harboring p λ P R pckA increased 2.5 fold. In the case of p λ P R ppsA pckA, the activities of PpsA and PckA increased 3.1 fold, and 2.3 fold respectively, and the yield of 3 deoxy α arabinoheptulosonate 7 phosphate (DAHP) increased 2.1 fold, which was higher than that with only a single gene." @default.
- W2364777640 created "2016-06-24" @default.
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- W2364777640 date "2002-01-01" @default.
- W2364777640 modified "2023-09-25" @default.
- W2364777640 title "Cloning and Co-expression of pps A and pck A Genes in Escherichia coli" @default.
- W2364777640 hasPublicationYear "2002" @default.
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