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- W2364789596 abstract "Objective To study the function of luxS gene relating to quorum sensing system and synthesize AI-2 signaling molecules in vitro and to further explore the LuxS/AI-2 dependent quorum sensing system in Enterococcus faecalis.Methods First,Vibrio harveyi BB170 was used as a reporter strain to detect the AI-2 activity of culture supernatants from E.faecalis.Secondly,luxS and pfs genes were amplified from E.faecalis and compared with the amino acid sequences.Then,the two genes were inserted into pGEX-4T-1 expression vector,transformed into Escherichia coli BL21(DE3) for expression and purification.Finally,AI-2 was synthesized in vitro with S-adenosylhomocysteine(SAH) as a substrate and the purified proteins or cell proteins from E.coli as catalytic enzymes for the reaction.Results Three strains of E.faecalis were all able to secrete the active AI-2 signaling molecules.A high LuxS protein homology was analyzed among E.faecalis,V.harveyi,Salmonella typhimurium and E.coli.The active AI-2 molecules could be synthesized by SAH with purified proteins or cell proteins from E.coli.Conclusion LuxS/AI-2 dependent quorum sensing system is found in E.faecalis and the luxS gene is required for AI-2 production." @default.
- W2364789596 created "2016-06-24" @default.
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- W2364789596 date "2011-01-01" @default.
- W2364789596 modified "2023-09-28" @default.
- W2364789596 title "Function analysis of luxS gene relating to quorum sensing system in Enterococcus faecalis" @default.
- W2364789596 hasPublicationYear "2011" @default.
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