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- W2364791816 abstract "Objective: To obtain the foreign DNA to cloning is introduced in this paper. As foreign DNAs, human angiogenin gene fragments(hAngf) were obtained with PCR and cloning for research. Methods: The PCR technique in which the primers were used as template each other was adopted to obtain the foreign DNAs for Cloning. The PCR products become into inserts after digested by restriction endonuclease and then they were inserted into pBluescript vectors to be cloned. Because of the different length of nucleotide sequences between containing the insert or not after treated by several restriction endonucleases, the positive clones and Ang-recombinants were screened and finally they were identified with electrophoresis. Results: The hAng inserts were obtained successfully by PCR and the electrophoretogram showed that the hAngf -pBluescripts wanted were obtained by the means properly adopted. Conclusion: Enough human angiogenin gene fragments(foreign DNAs) were synthesized by PCR and then they were inserted into vectors to be recombined in vitro. The studies of clinical applications and other areas about human angiogenin will be able to be carried out." @default.
- W2364791816 created "2016-06-24" @default.
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- W2364791816 date "2000-01-01" @default.
- W2364791816 modified "2023-09-23" @default.
- W2364791816 title "A simple convenient and specific way to obtain foreign DNAs for cloning" @default.
- W2364791816 hasPublicationYear "2000" @default.
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