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- W2364792836 abstract "Objective: In order to explore the function and distribution of auxin efflux carrier protein OsPIN1a in different tissues of rice. Method: The Japonica rice Zhonghua 11 and its over- expressed OsPIN1a gene strains with GFP and GUS reporter gene were chosen as materials. PCR method was used to detect the hygromycin resistance gene marker for transgenetic plants,semi- quantitative RT- PCR was used to detect the expression level of OsPIN1a in rice root,leaf and panicle,the GUS activity in rice root,leaf and panicle was detected by using GUS staining,and subcellular localization of OsPIN1a- GFP in the root tip observed by a laser confocal- microscopy. Result: The results showed that all of transgenic strains were positive by genomic PCR detection; the hygromycin resistance gene marker and OsPIN1a gene both expressed in rice roots,leaves and panicles by semi- quantitative RT- PCR. By GUS activity staining we found that panicles, leaves and roots all displayed GUS activity. Besides,the expression of GUS were stronger in young tissues,such as the newly- germinated seeds,coleoptile and stigma. The GUS activity were also induced by IAA and suppressed by polar auxin transport inhibitors( TIBA). Moreover,the OsPIN1a- GFP protein mainly located on the cell membrane in rice root tips by a laser confocal- microscopy. Conclusion: The experimental results showed that OsPIN1a gene might involve in the development and morphogenesis of these tissues by polar auxin transport." @default.
- W2364792836 created "2016-06-24" @default.
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- W2364792836 date "2014-01-01" @default.
- W2364792836 modified "2023-09-23" @default.
- W2364792836 title "Research on Expression and Localization of OsPIN1a Gene in Different Tissues in Rice" @default.
- W2364792836 hasPublicationYear "2014" @default.
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