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- W2364795757 abstract "A glutamate decarboxylase(GAD)-producing lactic acid bacteria(LAB) strain HS2 was isolated from pickled vegetable.When the biotransformation was conducted in cells with strain HS2 for 1 h,the content of γ-aminobutyric acid in biotransformation solution of strain HS2 was(13.114 ± 0.133) g/L.Base on morphological,physiological and biochemical characteristics,16S rDNA and phylogenic analysis,the strain HS2 was identified as Pediococcus pentosaceus.The optimal temperature and pH for GAD activity were 35 ℃ and 4.5.The GAD from strain HS2 was stable at 50 ℃ for 4 h and resistant to pH in the range of 4.0-6.5.In addition,calcium ions could result in a significant increase of GAD activity,which resulted in an enhancement of GAD activity by 37.21% and 23.02% at the concentrations of 5 mmol/L and 50 mmol/L Ca2+.Similarly,Mg2+ and Mn2+ also could increase GAD activity at the concentration of 50 mmol/L,whereas Co2+ could improve GAD activity at the concentration of 5 mmol/L." @default.
- W2364795757 created "2016-06-24" @default.
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- W2364795757 date "2010-01-01" @default.
- W2364795757 modified "2023-09-28" @default.
- W2364795757 title "Identification and Enzymological Characterization of Glutamate Carboxylase-producing Pediococcus" @default.
- W2364795757 hasPublicationYear "2010" @default.
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