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- W2364796074 abstract "The paper studied the viability determination of two kinds of algae Anabae na flos-aquae and Microcystis aeruginosa by FDA-PI double staining. Fluo rescence emission from cells was measured by fluorescence microscopy. The result s indicated that viable and dead cells were stained as bright green and red fluo rescent cells respectively by fluorescein diacetate and propidium iodide under b lue excitation(495nm). The staining rate has relation with the cell density. If the cell density of Anabaena flos-aquae and Microcystis aeruginosa was 6×10~7—7×10~9cells·l~-1 and 4×10~7—5×10~8cel ls·l~-1 , the FDA staining rate would be more than 94% and 91%. But if the cell density of Anabaena flos-aquae increased to 5×10~9 cells·l~ -1 ,the staining rate would fall to 67% obviously due to cell twisting togethe r. The staining rate of PI can reach 100% for the two kinds of algae. Based on t he fluorescent light from cells, it can be easy to determinate the cell viabilit y. FDA-PI double staining method is a new method to measure the algae cell viability." @default.
- W2364796074 created "2016-06-24" @default.
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- W2364796074 date "2005-01-01" @default.
- W2364796074 modified "2023-09-25" @default.
- W2364796074 title "THE VIABILITY DETERMINATION OF CYANOBACTERIA BY DOUBLE STAINING WITH FLUORESCEIN DIACETATE AND PROPIDIUM IODIDE" @default.
- W2364796074 hasPublicationYear "2005" @default.
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