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- W2364796535 abstract "Object To improve the HPLC method for determination of ferulic acid (FA) and to quantitate serum FA concentration in patients with blood stasis after oral administration of decoction of Ligusticum chuanxiong Hort and Salviave miltiorrhizae Bunge root Methods The serum sample was treated with a boiling water bath The coumarin was used as internal standard compound The mixed solvent consisted of methanol, water and acetic acid (40∶59 7∶0 3) was used as elutriant The flow rate was 1 0 mL/min The Diamonsil TM column (150 mm×4 6 mm, 5 μm) was used FA was detected at UV 322 nm and the peak of FA in serum was recognized by photodiode array detector Results FA and internal standard compound were separated completely under the condition described as above FA showed a good linearity in the range of 32 18～965 38 ng/mL (r=0 998 1) The RSD was less then 10%, and the minimum detection amount was 1 6 ng/mL and the minimum detection concentrations in serum was 15 7 ng/mL Conclusion Improved HPLC method is simple, sensitive, rapid and accurate It can be applied to detect FA concentration in human serum" @default.
- W2364796535 created "2016-06-24" @default.
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- W2364796535 date "2002-01-01" @default.
- W2364796535 modified "2023-09-25" @default.
- W2364796535 title "Quantitative analysis of ferulic acid in serum after oral administraiton of decoction of both Ligusticum chuanxiong and Salviave miltiorrhizae root in patients with blood stasis by HPLC" @default.
- W2364796535 hasPublicationYear "2002" @default.
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