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- W2364797955 abstract "This paper is aimed to construct the eukaryotic expression plasmid of the fusion gene of α-gal mimic epitope and GPI anchored sequence,and to study the expression of tumor-specific anchored α-gal mimic epitope as well as its cytolysis effect in A549 cells. The gal-GPI fusion gene was amplified by PCR,then the pSGFP-gal-GPI eukaryotic expression plasmid was constructed,which was modulated by tumor-specific survivin promoter. Then the recombinant plasmid was transfected into A549 cells by Lipofec-tamine 2000. Fluorescence microscopy showed that the fluorescence of transfected A549 distributed around the cell membrane unevenly; immunocytochemistry indicated the recombinant protein was around the A549 cell membrane; while Western blotting confirmed that the protein was successfully expressed in A549 cells,which can react with human IgG in serum. Furthermore,the transfected A549 cells could react with normal human serum (anti-gal) and cause the cytolysis effect induced by complement. In this study,the pSGFP-gal-GPI eukaryotic expression plasmid which can express anchored α-gal mimic epitope on A549 cell was constructed successfully,which provide a basis for further study of targeting gene therapy of tumor." @default.
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- W2364797955 date "2010-01-01" @default.
- W2364797955 modified "2023-09-26" @default.
- W2364797955 title "Construction and function research of eukaryotic expression plasmid encoding tumor-specific anchored α-gal mimic epitope" @default.
- W2364797955 hasPublicationYear "2010" @default.
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