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• W236482162 abstract "alterations. These changes include establishing non-nitrogen-fixing symbiosis with clover, doubled production of exopolysaccharide (EPS) with elevated content of the high molecular weight (HMW) form, and EPS viscosity significantly higher than in the wild type strain. Moreover, changes in the LPS I form containing the O antigen were found by SDS-PAGE analysis. The absence of fucose, 2-O-methyl–6-deoxyhexose and 3-N-methyl–3,6-dideoxyhexose, and the appearance of two other 6-deoxyhexoses, confirmed changes in the LPS O polysaccharide of the pssB mutant. These alterations indicate that the pssB mutation greatly affected not only EPS processing but also biosynthesis of the O antigen of LPS. Light and electron microscopy revealed that clover nodules infected with the pssB mutant accumulated significantly increased quantities of starch throughout the nodule and overproduced empty membranous structures, suggesting a defect in bacterial release into plant cells." @default.
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• W236482162 date "2007-01-01" @default.
• W236482162 modified "2023-10-11" @default.
• W236482162 title "Effects of PSSB mutation on surface Polysaccharides and symbiotic phenotype of rhizobium leguminosarum BV. trifolii" @default.
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