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- W2364854673 abstract "Objective To establish a method of loop-mediated isothermal amplification for rapidly detecting Streptococcus pneumoniae in sputum samples.Methods Four S.pneumoniae-specific LAMP primers which be aimed at the gene of autolysin(lytA) were designed from the published sequence of strain R6,then detected Streptococcus pneumoniae in sputum samples by LAMP method and detected that by traditional and PCR methods at the same time.Results 54 samples were detected in our experiment and the traditional,PCR and LAMP methods have 27,31 and 36 positive test results respectively.Conclusion In spite of the three methods in our experiment have no significant differences,the LAMP technique is more rapid and convenient compared with PCR method and traditional culture method,so this method is still very useful for the rapid detection of S.pneumoniae." @default.
- W2364854673 created "2016-06-24" @default.
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- W2364854673 date "2009-01-01" @default.
- W2364854673 modified "2023-09-25" @default.
- W2364854673 title "The detection of Streptococcus pneumoniae in sputum samples by loop-mediated isothermal amplification method" @default.
- W2364854673 hasPublicationYear "2009" @default.
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