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- W2364862481 abstract "Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.The sequencing results indicated that the cDNA gene coded for chymosin B and had 1 292 nt which coded 381 amino acid residues.The sequence of amino acids included a predicted signal peptide region(16 amino acids) and a prochymosin region(42 amino acids).Comparison of the Xinong Saanen Goat preprochymosin sequence with those of the goat,sheep and cattle revealed 99.41%,98.74%,95.29% and 99.21%,98.42%,94.22% identity at the nucleotide and amino acid level,respectively." @default.
- W2364862481 created "2016-06-24" @default.
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- W2364862481 date "2007-01-01" @default.
- W2364862481 modified "2023-09-26" @default.
- W2364862481 title "Cloning and sequence analyses of preprochymosin cDNA of Xinong Saanen Goat" @default.
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