Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364866093> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2364866093 abstract "The purine nucleoside phosphorylase(EC 2.4.2.1 PNPase)gene of Enterobacter aerogenes 、Erwinia carotovora and E.coli was amplified by polymerase chain reaction(PCR).The amplified fragment with about 720bp was cloned into the prokaryotic expression vector pET-28b(+).The three recombinant expression vectors that contained the target fragment were identified by digestion using restriction endonucleases and named as pP-8,pLErO-2 and pD1-1.The nucleotides and deduced amino acid sequences of the cloned PNPase gene from three strains were analyzed.The results showed that the gene had a relatively high homology with the corresponding gene from Enterobacteriaceae.The construction of recombinant PNPase will provide a basis for the research on biocatalysed synthesis of nucleosides and modified nucleosides." @default.
- W2364866093 created "2016-06-24" @default.
- W2364866093 creator A5013697180 @default.
- W2364866093 creator A5044240389 @default.
- W2364866093 date "2006-01-01" @default.
- W2364866093 modified "2023-09-25" @default.
- W2364866093 title "Cloning and expression of purine nucleoside phosphorylase in Escherichia coli" @default.
- W2364866093 hasPublicationYear "2006" @default.
- W2364866093 type Work @default.
- W2364866093 sameAs 2364866093 @default.
- W2364866093 citedByCount "0" @default.
- W2364866093 crossrefType "journal-article" @default.
- W2364866093 hasAuthorship W2364866093A5013697180 @default.
- W2364866093 hasAuthorship W2364866093A5044240389 @default.
- W2364866093 hasConcept C104317684 @default.
- W2364866093 hasConcept C121050878 @default.
- W2364866093 hasConcept C153911025 @default.
- W2364866093 hasConcept C181199279 @default.
- W2364866093 hasConcept C185592680 @default.
- W2364866093 hasConcept C199360897 @default.
- W2364866093 hasConcept C2776476023 @default.
- W2364866093 hasConcept C2776735402 @default.
- W2364866093 hasConcept C40767141 @default.
- W2364866093 hasConcept C41008148 @default.
- W2364866093 hasConcept C547475151 @default.
- W2364866093 hasConcept C55493867 @default.
- W2364866093 hasConcept C71615608 @default.
- W2364866093 hasConcept C86803240 @default.
- W2364866093 hasConceptScore W2364866093C104317684 @default.
- W2364866093 hasConceptScore W2364866093C121050878 @default.
- W2364866093 hasConceptScore W2364866093C153911025 @default.
- W2364866093 hasConceptScore W2364866093C181199279 @default.
- W2364866093 hasConceptScore W2364866093C185592680 @default.
- W2364866093 hasConceptScore W2364866093C199360897 @default.
- W2364866093 hasConceptScore W2364866093C2776476023 @default.
- W2364866093 hasConceptScore W2364866093C2776735402 @default.
- W2364866093 hasConceptScore W2364866093C40767141 @default.
- W2364866093 hasConceptScore W2364866093C41008148 @default.
- W2364866093 hasConceptScore W2364866093C547475151 @default.
- W2364866093 hasConceptScore W2364866093C55493867 @default.
- W2364866093 hasConceptScore W2364866093C71615608 @default.
- W2364866093 hasConceptScore W2364866093C86803240 @default.
- W2364866093 hasLocation W23648660931 @default.
- W2364866093 hasOpenAccess W2364866093 @default.
- W2364866093 hasPrimaryLocation W23648660931 @default.
- W2364866093 hasRelatedWork W1479724966 @default.
- W2364866093 hasRelatedWork W1484124656 @default.
- W2364866093 hasRelatedWork W1541512177 @default.
- W2364866093 hasRelatedWork W1585995364 @default.
- W2364866093 hasRelatedWork W1675979998 @default.
- W2364866093 hasRelatedWork W1971742369 @default.
- W2364866093 hasRelatedWork W1982710856 @default.
- W2364866093 hasRelatedWork W1995616206 @default.
- W2364866093 hasRelatedWork W2012058711 @default.
- W2364866093 hasRelatedWork W2015167700 @default.
- W2364866093 hasRelatedWork W2015438746 @default.
- W2364866093 hasRelatedWork W2041337849 @default.
- W2364866093 hasRelatedWork W2041735993 @default.
- W2364866093 hasRelatedWork W2052273588 @default.
- W2364866093 hasRelatedWork W2056146918 @default.
- W2364866093 hasRelatedWork W2078681663 @default.
- W2364866093 hasRelatedWork W2113648685 @default.
- W2364866093 hasRelatedWork W2114398315 @default.
- W2364866093 hasRelatedWork W2160519658 @default.
- W2364866093 hasRelatedWork W2165563653 @default.
- W2364866093 isParatext "false" @default.
- W2364866093 isRetracted "false" @default.
- W2364866093 magId "2364866093" @default.
- W2364866093 workType "article" @default.