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- W2364875694 abstract "In order to improve the enzyme activity of Escherichia coli Rosetta(DE3)-pET-22-bgl-(kil-Km),the effects of carbon sources,nitrogen sources and C/N ratio on the production of β-1,3-1,4-glucanase H(A107-M) by E.coli Rosetta(DE3)-pET-22-bgl-(kil-Km) were investigated by one-factor-a-time experiment.Box-Behnken design and a response surface methodology(RSM) were further used to optimize the above critical factors for enzyme production.The optimized medium contained(g/L):glycerol 10.01,casein peptone 12.03,yeast 24,NaCl 10,(NH4)2SO4 4.77,KH2PO4 2.31,and K2HPO4 12.54.When the recombinant E.coli was cultivated in the optimized medium for 27 h,the enzyme activity of β-1,3-1,4-glucanase H(A107-M) reached 78.32 U/mL(lichenan as the substrate),which was about 2.84 times of which obtained in the initial medium." @default.
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- W2364875694 date "2011-01-01" @default.
- W2364875694 modified "2023-09-25" @default.
- W2364875694 title "Medium Optimization for β-1,3-1,4-Glucanase Production by Recombinant Escherichia coli" @default.
- W2364875694 hasPublicationYear "2011" @default.
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