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- W2364884295 abstract "To establish a new, real time, dynamic and direct optical detection method for mast cell degranulation caused by anaphylactoid reaction.A CD63-GFP plasmid was constructed and introduced steadily into rat basophilic leukemia (RBL-2H3) cells. The movements of CD63-GFP, which was located on both the granule membranes and the plasma membranes of RBL cells stimulated by Compound 48/80, were studied by confocal laser scanning microscope (CLSM) and total internal reflection fluorescence microscope (TIRFM) both inside and on the surface of living RBL-2H3 cells.Before antigen stimulation, most granules with CD63-GFP hardly moved in RBL cells. However, after antigen stimulation, the granules moved dramatically. They reached the plasma membranes in a few minutes and fused with them instantaneously. The velocity of the granule movement toward the plasma membranes on antigen stimulation was calculated to be 0.05 micron x s(-1).Analysis of the movement of each granule provided a new insight into the elementary process of degranulation. The method is rapid, sensitive and reliable, which could be used as a new detection method for anaphylactoid reaction in vitro." @default.
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- W2364884295 date "2011-07-15" @default.
- W2364884295 modified "2023-09-26" @default.
- W2364884295 title "Real-time detection of mast cell degranulation in anaphylactoid reaction" @default.
- W2364884295 doi "https://doi.org/10.4268/cjcmm20111405" @default.
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