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- W2364885613 abstract "The aim of this study is to develop a duplex RT-PCR method which can simultaneously detect Peanut stripe virus(PStV) and Cucumber mosaic virus(CMV).The reverse transcript of the total RNA extracted from diseased peanut leaves were used as templates for RT-PCR.The specific primers for duplex RT-PCR were designed according to conserved regions of the published sequences of PStV and CMV.The specificity and sensitivity of this duplex RT-PCR assay were tested.A duplex RT-PCR method for the detection of PStV and CMV was successfully established.There were two specific bands amplified in this system,one was 1054 bp refer to CMV,the other one was 300 bp refer to PStV.Sequence analysis of the amplified products showed that there were high homology of PStv subgroup(93%～99%)and CMV(90%～99%)with the sequences of PStV subgroup and CMV in GenBank.The results indicated that this duplex RT-PCR method can simultaneously detect PStV and CMV with high stability and specificity." @default.
- W2364885613 created "2016-06-24" @default.
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- W2364885613 date "2012-01-01" @default.
- W2364885613 modified "2023-09-23" @default.
- W2364885613 title "Detection of PStV and CMV by a Duplex RT-PCR Method" @default.
- W2364885613 hasPublicationYear "2012" @default.
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