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- W2364887396 abstract "Objective The study on nervous system infection requires us to obtain microglia with high purity and biological status. However,present methods for microglia purification can not meet these requirements. It is necessary to establish an efficient separation method. Methods After having prepared the whole mouse brain cell suspension, two-step magnetic activated cell positive sorting (MACS) technique was applied specifically for CD11b+ microglia sorting. The purity of the cells was detected by flow cytometry, the morphology of the cells was observed by Wright-Giemsa staining, and the viability of the cells were determined by 7-AAD staining. Results The study obtained mice microglia with purity of (59.98 ± 13.61)% for Step 1 MACS and (97.62 ± 1.35)% for Step 2 MACS, which could bear good viability and morphology. Conclusions The two-step magnetic cell sorting technique could obtain high purity microglia stably, with no significant effect on its activity and morphology. The method suits for studying the infection in CNS." @default.
- W2364887396 created "2016-06-24" @default.
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- W2364887396 date "2012-01-01" @default.
- W2364887396 modified "2023-09-26" @default.
- W2364887396 title "Purification of microglia by two-step magnetic activated cell sorting" @default.
- W2364887396 hasPublicationYear "2012" @default.
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