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- W2364888053 abstract "Objective To investigate the inhibition of lysozyme on ceftazidime induced lipopolysaccharide release. Methods Ceftazidime (CAZ), a β lactam antibiotic, was exposed to Es cherichia coli O111:B4, Salmonella typhimurium LT2 or E. coli J5 at a final concentration of 50μg/ml in the presence or absence of lysozyme. The culture supernatants were collected at 3h after addi tion of ceftazidime. The concentrations of LPS in the supernatants and tumor necrosis factor α(TNF α) or interleukin 6 (IL 6) productivity of the supernatants were tested. Results CAZ caused a rapid lysis of bacteria and high concentrations of LPS release into culture supernatants. The supernatants induced high level of TNF α and IL 6 production in macrophages and in mouse sera. Egg white lysozyme in combination with CAZ showed bactericidal activity but without bacteriolysis, resulting in a decreased LPS concentration in the culture supernatant as well as a low proinflammatory cytokine productivity. Conclusion Lysozyme may inhibit ceftazidime induced LPS release from bacteria." @default.
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- W2364888053 date "1999-01-01" @default.
- W2364888053 modified "2023-09-25" @default.
- W2364888053 title "Inhibition of ceftazidime induced lipopolysaccharide release by egg white lysozyme" @default.
- W2364888053 hasPublicationYear "1999" @default.
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